dextran hydrodynamic radius

Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. The genus Weissella: taxonomy, ecology and biotechnological potential. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). eCollection 2015. Careers. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Microporation was performed 2 times on each sample. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. @ Nilimesh See attached. (2013). Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Eng. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Proc of SPIE. Catiker, E.; Guner, A. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Dextran produced from Weissella cibaria JAG8 was purified and characterized. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Privacy Biomaterials, 22, 1891-1898. Nordmeier, Eckliard (1993). Elias, Hans-Georg (1975). Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. dextran hydrodynamic radius 2016;9706:97061E6. statement and Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. All branched polysaccharide types could be described approximately by the hyperbranching theory. On the molecular weight distribution of dextran T-500. 2003;10:33847. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Disclaimer. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. J. Phys. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Drug Deliv. Pharmaceut Sci Tech. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. One day before the experiment, the skin was stabilized in the skin culture medium. Terms and Conditions, Banga AK. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. volume24, Articlenumber:22 (2020) Synthesis and characterization of blue dextrans. Radiofrequency ablation of malignant liver tumors. 20K. * shows a significant difference between the M.W. What is hydrodynamic radius The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. The human skins were from the back or thigh of a 24-year-old woman. 8600 Rockville Pike Ubbelohde, Leo. Indo AM J Pharm. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. 1994, 95-0355. Small Intestinal Permeability and Gut-Transit Time Determined Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Masuelli, Martin Alberto (2013). The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. Dilute solution properties and degree of chain branching Pharm Res. 10K, and c M.W. ; Rjumtsev, E.I. 2005;22(4):5505. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. Doke SK, Dhawale SC. Journal of Applied Polymer Science, 27, 795-810. ACS Nano. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. 2009;301(8):60913. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. (2008). Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. (1998). official website and that any information you provide is encrypted The Hydrodynamic Radii of Macromolecules and Their Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in FOIA Ibrahim O, Munavalli GS, Dover JS. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. Format). The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. (2012). Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Amersham Biosciences, AA Edition, www.amershambiosciences.com. 4D imaging reveals mechanisms of clay-carbon protection and 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. Advances in Cosmetic Surgery. 2015;23(3):2239. Experimental and numerical study on the mixed lubrication (2007). Macromolecules. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. The mechanism of the dextran-induced red blood cell Aggregation. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Hydrodynamic Microporation applications for enhancing drug delivery. dextran hydrodynamic The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. The solution was measured using an Anton Paar DMA35N densitometer. A single frequency laser is directed to the sample contained in a cuvette. In addition, we evaluated the regeneration of SC after microporation. (2007). The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. The Mark-Houwink. Smith, David R.; Raymonda, John W. (1972). MeSH terms The receptor chamber was filled with PBS. Int J Food Sci Nutr. Hrabchak C, Flynn L, Woodhouse KA. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. ; Veldhuis, T.F.J. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. PubMedGoogle Scholar. Chen Rong Huei; Min Lang Tsai (1998). J Pharm Sci. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. ; Aberle, T; Burchard, W. (2000). Unauthorized use of these marks is strictly prohibited. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. 7 8 WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Recent Pat Drug Deliv Formul. WebTry out PMC Labs and tell us what you think. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Dextran (2003). (2013). For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Food Funct. Recent Pat. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Influence of molecular weight on AATEX. Journal of Physical Chemistry. Probiotics Antimicrob Proteins. II. For Research Use Only. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. dextran hydrodynamic radius Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. 1984;17(6):11703. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. dextran Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them.